Simple spectrophotometric method for estimation of drugs using chloraminet and indigo caramine dye couple

The five drugs viz., GEM, PHE, TRD, IMT, VRP were estimated using a simple, specific, accurate and precise UV–visible spectrophotometric method. These methods involve the addition of a known nexcess of Chloramine-T to the drugs in acid medium followed by estimation of residual Chloramine-T by reacting with a fixed amount of Indigo caramine dye and measuring the absorbance of the dye at 608 nm. The calibration curves obeyed Beer’s law over the concentration range of 3.0,5.5,6.5 μg mL-1 of GEM; 2.5,3.69,5.01 μg mL-1 of PHE ; 2.0,3.5,7.5 μg mL-1 of TRD; 3.0,4.5,7.0 μg mL- 1 of IMT; and 4.2,5.5,7.0 μg mL-1 of VRP were chosen. The methods that are developed were found to be suitable for the estimation of these drugs in bulk and their formulations. Several parameters such as linearity, accuracy, and precision, Limit of detection and Limit of quantification were considered for the analysis of results of the drugs and their corresponding validation were carried out statistically.

GEM is chemically 7-((4Z)-3-(aminomethyl)-4-methoxyiminopyrrolidin- 1-yl]-1- cyclopropyl-6-fluoro-4-oxo-1, 4 dihydro-1, 8- napththyridine-3-carboxylic acid. It has high affinity towards bacterial topoisomerase IV and is a fourthgeneration fluoroquinolone antibacterial agent. Owing to its anti-bacterial activity to both gram-positive and gramnegative, this compound is mainly used for the treatment of acute exacerbations of urinary tract infection, chronic bronchitis and pneumonia. This drug is not official in any pharmacopoeia. Analysis of the literature demonstrated that there are numerous analytical methods for the estimation of GEM is specified. The present work mainly aims to describe a simple and sensitive UV Phenylephrine hydrochloride ((R)-1-(3-hydroxyphenyl) 2- (methylamino) ethanol hydrochloride, C9H13O2N. HCl], is a white crystalline powder, andbelongs to the group of medicines called sympathomimetics. It acts as a stimulator for alpha receptors in certain areas of the body. It is used locally, as decongestant, for non-specific and allergic conjunctivitis, sinusitis and nasopharyngitis.

A novel, simple, accurate and precise method, which is applicable in ordinary laboratories was developed for the determination of GEM, PHE, TRD, IMT, VRP. Using this method, the drugs could be determined from frequently used excipients without intrusion, in tablet formulation. In this method, inexpensive and simple to prepare solvents were used. They could be used for routine drug analysis in a quality control laboratory.

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