From the perspective of energy metabolism, the study's goal was to clarify the stearoyl-coenzyme a desaturase (SCD1)-dependent gene network of c9, t11-CLA production in MAC-T cells. The cells were separated into the TVA group, the TVA group and the control group, and then treated with trans-11-octadecenoic acid (TVA), a pharmacological inhibitor of SCD1. The contents of c9, t11-CLA, and TVA were measured using gas chromatography. Real-time PCR was used to assess the mRNA levels of SCD1 and other candidate genes. To investigate the genes implicated in the SCD1-dependent c9, t11-CLA production, parallel reaction monitoring (PRM), tandem mass tag (TMT)-based quantitative proteomics, and small RNA interference were used.

The outcomes demonstrated that the production of c9, t11-CLA was prevented by the SCD1 deficit caused by CAY10566. TMT-based quantitative proteomics analysis was used to evaluate 60 SCD1-related proteins that are largely engaged in energy metabolism pathways. Furthermore, PRM analysis was used to validate 17 proteins. After the small RNA interference investigation, 11 genes' unfavourable associations with SCD1 were confirmed. Based on the aforementioned findings, we deduced that genes connected to pathways for energy metabolism have an effect on the molecular process of c9, t11-CLA production that is dependent on SCD1. Cis9, trans11-conjugated linoleic acid is a bioactive substance with a number of advantageous biological effects, one of which is a critical contribution to the enhancement of milk's nutritional value.

The SCD1-dependent gene network of mammary gland c9, t11-CLA production is currently poorly understood. In order to identify the individual differences in dairy cows' capacity for c9, t11-CLA synthesis, it is critical for further research to focus on the molecular mechanism of c9, t11-CLA synthesis. This appears to be a choice that might enhance the nutritional content of milk.